Terminator

Part:BBa_K3675003:Design

Designed by: João Costa   Group: iGEM20_NOVA_LxPortugal   (2020-10-22)


PPKT2440_T1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 10
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 109
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 100
    Illegal SapI site found at 57


Design Notes

The sequence was kept has described in the original work and it is meant to be used in a Pseudomonas Strain. It was already validated in Pseudomonas Putida KT2440


Source

This sequence was obtained from a work that screened for novel transcriptional tools for synthetic biology. The work has the title " Expanding the Toolbox of Broad Host-Range Transcriptional Terminators for Proteobacteria through Metagenomics " and DOI: https://doi.org/10.1021/acssynbio.8b00507

References

Vanesa Amarelle, Ananda Sanches-Medeiros, Rafael Silva-Rocha, and María-Eugenia Guazzaroni ACS Synthetic Biology 2019 8 (4), 647-654 DOI: 10.1021/acssynbio.8b00507